Axon initial segment volume changes

The proximal axon area of an axon shown in (A) was quantified in Neurolucida software (B), by calculating the volume of approximately the first 10 µm of axon from the soma. This volume was then divided by the actual length of the axon measured (varied from 8 - 13 µm) to obtain the cross sectional area. One day after the last evoked seizure, the cross sectional area of the proximal axon was significantly increased in kindled mice (D) relative to controls (C). Arrows delineate the portion of the